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日期：2024-08-02
IF：0.4標(biāo)準(zhǔn)曲線
| Concentration (ng/mL) | OD | Corrected OD |
|---|---|---|
| 900.00 | 0.183 | |
| 450.00 | 0.385 | |
| 225.00 | 0.575 | |
| 112.50 | 0.823 | |
| 56.25 | 1.157 | |
| 28.13 | 1.592 | |
| 14.07 | 1.624 | |
| 0.00 | 2.256 |
精密度
Intra-assay Precision (Precision within an assay)：CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)：CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
回收率
Matrices listed below were spiked with certain level of recombinant 5-HT and the recovery rates were calculated by comparing the measured value to the expected amount of 5-HT in samples.
| Matrix | Recovery range | Average |
|---|---|---|
| serum(n=5) | 88-103% | 95% |
| EDTA plasma(n=5) | 95-107% | 101% |
| Heparin plasma(n=5) | 80-95% | 93% |
線性
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of 5-HT and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
|---|---|---|---|---|
| serum(n=5) | 82-93% | 95-104% | 87-98% | 92-99% |
| EDTA plasma(n=5) | 89-99% | 83-96% | 98-104% | 88-101% |
| Heparin plasma(n=5) | 87-96% | 87-96% | 96-105% | 95-103% |
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