天天摸天天碰天天添中文字幕,国产区点击进入在线影院,无码人妻一区二区免费AV,亚洲精品国产av现线

產(chǎn)品中心

大鼠腫瘤壞死因子α(TNFa)酶聯(lián)免疫吸附檢測(cè)試劑盒
ELK1396
規(guī)格: 價(jià)格:
48T ¥1680.00
96T ¥2400.00

Overview 文獻(xiàn)

Product name: Rat TNFa(Tumor Necrosis Factor Alpha) ELISA Kit
Reactivity: Rat
Alternative Names: TNF Alpha; DIF; TNF-A; TNFSF2; Cachectin; Tumor Necrosis Factor Ligand Superfamily Member 2
Assay Type: Sandwich
Sensitivity: 6.1 pg/mL
Standard: 1000 pg/mL
Detection Range: 15.63-1000 pg/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 3.5h
Research Area: Cytokine;Tumor immunity;Infection immunity;
Uniprot ID: P16599
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat TNFa. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat TNFa. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat TNFa, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat TNFa in the samples is then determined by comparing the OD of the samples to the standard curve.

標(biāo)準(zhǔn)曲線

Concentration (pg/mL) OD Corrected OD
1000.00 2.345 2.256
500.00 1.769 1.680
250.00 1.225 1.136
125.00 0.917 0.828
62.50 0.521 0.432
31.25 0.366 0.277
15.63 0.207 0.118
0.00 0.089 0.000

精密度

Intra-assay Precision (Precision within an assay):CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays):CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant TNFa and the recovery rates were calculated by comparing the measured value to the expected amount of TNFa in samples.
Matrix Recovery range Average
serum(n=5) 80-90% 85%
EDTA plasma(n=5) 84-106% 100%
Heparin plasma(n=5) 87-93% 90%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TNFa and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 89-97% 85-94% 87-96% 85-97%
EDTA plasma(n=5) 85-94% 87-96% 92-101% 82-98%
Heparin plasma(n=5) 85-99% 98-106% 86-99% 78-95%
關(guān)閉

在線咨詢

Online consultation

  • 在線咨詢
  • 技術(shù)支持

關(guān)注微信公眾號(hào)

微信掃一掃立即咨詢

微信掃一掃立即咨詢

關(guān)閉
文安县| 翁源县| 东乡县| 杭锦后旗| 定西市| 浦江县| 井研县| 寿阳县| 邓州市| 福贡县| 昌图县| 南雄市| 云南省| 察哈| 通化市| 卫辉市| 彭州市| 萨嘎县| 沧源| 永吉县| 乐平市| 仙桃市| 信宜市| 廉江市| 光山县| 韶关市| 荆门市| 祁东县| 张家界市| 桐梓县| 九寨沟县| 渭南市| 平果县| 龙山县| 进贤县| 泗洪县| 乌兰察布市| 龙海市| 土默特右旗| 肇州县| 青河县|