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一步法大鼠雌二醇(E2)酶聯(lián)免疫吸附檢測試劑盒

ELK022ES

規(guī)格：	價格：
64T	￥2240.00
112T	￥3200.00

下載說明 ①

Overview

Product name:	EasyStep Rat E2(Estradiol) ELISA Kit
Reactivity:	Rat
Alternative Names:	17B-Estradiol; Oestradiol; Beta-Estradiol
Assay Type:	Competitive Inhibition
Sensitivity:	25 pg/mL
Standard:	1600 pg/mL
Detection Range:	25-1600 pg/mL
Sample Type:	Serum
Assay Length:	1.5h
Research Area:	Endocrinology;Reproductive science;Hormone metabolism;
Test principle:	This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat E2 antigen, and the Rat E2 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Rat E2 to each microplate well and incubated . After TMB substrate solution is added, the enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ±10nm. The concentration of Rat E2 in the samples is then determined by comparing the OD of the samples to the standard curve.

標(biāo)準(zhǔn)曲線

Concentration (pg/mL)	OD	Corrected OD
1600.00	0.211
800.00	0.389
400.00	0.655
200.00	0.895
100.00	1.119
50.00	1.522
25.00	1.762
0.00	2.101

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant E2 and the recovery rates were calculated by comparing the measured value to the expected amount of E2 in samples.

Matrix	Recovery range	Average
serum(n=5)	90-103%	96%
EDTA plasma(n=5)	82-95%	88%
Heparin plasma(n=5)	81-95%	88%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of E2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.