天天摸天天碰天天添中文字幕,国产区点击进入在线影院,无码人妻一区二区免费AV,亚洲精品国产av现线

產(chǎn)品中心

一步法人白介素6(IL6)酶聯(lián)免疫吸附檢測(cè)試劑盒
ELK001ES
規(guī)格: 價(jià)格:
64T ¥1960.00
112T ¥2800.00

Overview

Product name: EasyStep Human IL6(Interleukin 6) ELISA Kit
Reactivity: Human
Alternative Names: IL-6;MGI2-A; MGI2A; HGF; BSF2; HSF; IFNB2; B-Cell Stimulatory Factor-2; Hybridoma/Plasmacytoma Growth Factor; Hepatocyte Stimulating Factor; Cytotoxic T-Cell Differentiation Factor
Assay Type: Sandwich
Sensitivity: 0.1 pg/mL
Standard: 100 pg/mL
Detection Range: 1.56-100 pg/mL
Sample Type: serum, plasma
Assay Length: 1.5 h
Research Area: Cytokine;Tumor immunity;Infection immunity;Cardiovascular biology;
Uniprot ID: P05231
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IL6, and the Human IL6 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human IL6. After TMB substrate solution is added, only those wells that contain Human IL6 and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IL6 in the samples is then determined by comparing the OD of the samples to the standard curve.

標(biāo)準(zhǔn)曲線

Concentration (pg/mL) OD Corrected OD
100.00 2.999 2.939
50.00 2.373 2.313
25.00 1.683 1.623
12.50 1.117 1.057
6.25 0.708 0.648
3.13 0.344 0.284
1.57 0.175 0.115
0.00 0.060 0.000

精密度

Intra-assay Precision (Precision within an assay):CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays):CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant IL6 and the recovery rates were calculated by comparing the measured value to the expected amount of IL6 in samples.
Matrix Recovery range Average
serum(n=5) 90-105% 97%
EDTA plasma(n=5) 95-110% 100%
Heparin plasma(n=5) 93-102% 98%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL6 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 85-98% 98-110% 99-116% 95-110%
EDTA plasma(n=5) 87-102% 85-95% 85-98% 82-95%
Heparin plasma(n=5) 88-102% 95-106% 95-108% 96-115%

產(chǎn)品成分表

關(guān)閉

在線咨詢

Online consultation

  • 在線咨詢
  • 技術(shù)支持

關(guān)注微信公眾號(hào)

微信掃一掃立即咨詢

微信掃一掃立即咨詢

關(guān)閉
武冈市| 营口市| 鞍山市| 乌拉特前旗| 丹阳市| 台州市| 垣曲县| 长武县| 阳谷县| 梁山县| 岳西县| 龙江县| 探索| 禄丰县| 贵港市| 徐水县| 湘潭县| 泰和县| 长春市| 阿合奇县| 广汉市| 北宁市| 富顺县| 鸡西市| 贵南县| 衡山县| 鄯善县| 福鼎市| 甘泉县| 灯塔市| 龙山县| 咸阳市| 清徐县| 永新县| 龙胜| 新安县| 石棉县| 手游| 化州市| 蓬溪县| 申扎县|